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验证数据展示

  • WB analysis using 68446-1-Ig (same clone as 68446-1-PBS)

    WB analysis using 68446-1-Ig (same clone as 68446-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

    IF Staining of MCF-7 using 68446-1-Ig (same clone as 68446-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBP1 antibody included
  • IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    IHC staining of mouse kidney using 68446-1-Ig (same clone as 68446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68446-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FBP1 antibody included

FBP1 Monoclonal antibody, PBS Only

FBP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68446-1-PBS

产品说明书

CloneNo. 2G4E4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FBP1 Ready-To-Use IHC Kit. FBP1 primary antibody included.

产品信息

68446-1-PBS targets FBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原

CatNo: Ag3837

Product name: Recombinant human FBP1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-338 aa of BC012927

Sequence: MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 fructose-1,6-bisphosphatase 1
别名 FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1
计算分子量 338 aa, 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC012927
基因名称 FBP1
Gene ID (NCBI) 2203
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09467
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).

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