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验证数据展示

  • IF Staining of Hela using CL488-82897-2

    IF Staining of Hela using CL488-82897-2

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 FANCM antibody (CL488-82897-2, Clone: 230233G5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-82897-2

    FC experiment of HeLa using CL488-82897-2

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated FANCM Recombinant antibody (CL488-82897-2, Clone:230233G5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated FANCM Recombinant monoclonal antibody

FANCM Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82897-2

产品说明书

CloneNo. 230233G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 82897-2-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82897-2 targets FANCM in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag34447

Product name: Recombinant human FANCM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1481-1679 aa of BC140776

Sequence: VARKFLDDEAELSEEDAEYVSSDENDESENEQDSSLLDFLNDETQLSQAINDSEMRAIYMKSLRSPMMNNKYKMIHKTHKNINIFSQIPEQDETYLEDSFCVDEEESCKGQSSEEEVCVDFNLITDDCFANSKKYKTRRAVMLKEMMEQNCAHSKKKLSRIILPDDSSEEENNVNDKRESNIAVNPSTVKKNKQQDHCL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fanconi anemia, complementation group M
别名 Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM
GenBank蛋白编号BC140776
基因名称 FANCM
Gene ID (NCBI) 57697
RRIDAB_3673103
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IYD8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FANCM antibody CL488-82897-2Download protocol
IF protocol for CL Plus 488 FANCM antibody CL488-82897-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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