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验证数据展示

  • IP experiment of HepG2 using 82897-2-RR (same clone as 82897-2-PBS)

    IP experiment of HepG2 using 82897-2-RR (same clone as 82897-2-PBS)

    IP result of anti-FANCM (IP:82897-2-RR, 4ug; Detection:82897-2-RR 1:500) with HepG2 cells lysate 400 ug. This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

    IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

    IHC staining of mouse testis using 82897-2-RR (same clone as 82897-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82897-2-RR (FANCM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 82897-2-RR (same clone as 82897-2-PBS)

    FC experiment of PC-3 using 82897-2-RR (same clone as 82897-2-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug FANCM Recombinant antibody (82897-2-RR, Clone:230233G5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82897-2-RR (same clone as 82897-2-PBS)

    IF Staining of HeLa using 82897-2-RR (same clone as 82897-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCM antibody (82897-2-RR, Clone: 230233G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82897-2-PBS in a different storage buffer formulation.

FANCM Recombinant monoclonal antibody, PBS Only

FANCM Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 82897-2-PBS

产品说明书

CloneNo. 230233G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82897-2-PBS targets FANCM in IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34447

Product name: Recombinant human FANCM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1481-1679 aa of BC140776

Sequence: VARKFLDDEAELSEEDAEYVSSDENDESENEQDSSLLDFLNDETQLSQAINDSEMRAIYMKSLRSPMMNNKYKMIHKTHKNINIFSQIPEQDETYLEDSFCVDEEESCKGQSSEEEVCVDFNLITDDCFANSKKYKTRRAVMLKEMMEQNCAHSKKKLSRIILPDDSSEEENNVNDKRESNIAVNPSTVKKNKQQDHCL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fanconi anemia, complementation group M
别名 Fanconi anemia-associated polypeptide of 250 kDa, Fanconi anemia group M protein, FAAP250, EC:3.6.4.13, ATP-dependent RNA helicase FANCM
GenBank蛋白编号BC140776
基因名称 FANCM
Gene ID (NCBI) 57697
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IYD8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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