• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84150-2-RR

    WB analysis using 84150-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84150-2-RR (FAM136A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 84150-2-RR

    IHC staining of mouse testis using 84150-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 84150-2-RR

    IHC staining of mouse testis using 84150-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84150-2-RR

    IF Staining of HeLa using 84150-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FAM136A antibody (84150-2-RR, Clone: 241433D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of U2OS using 84150-2-RR

    FC experiment of U2OS using 84150-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug FAM136A Recombinant antibody (84150-2-RR, Clone:241433D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84150-2-RR

    Affinity and Kinetic Characterization of 84150-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84150-2-RR against Human FAM136A were performed. The affinity constant is below 1 pM.

FAM136A Recombinant antibody

FAM136A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84150-2-RR

产品说明书

CloneNo. 241433D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

241433D12, Protein FAM136A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HeLa cell, Jurkat cell, MCF-7 cell, U2OS cell, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84150-2-RR targets FAM136A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FAM136A fusion protein Ag34058 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 family with sequence similarity 136, member A
别名 241433D12, Protein FAM136A
观测分子量12 kDa
GenBank蛋白编号BC014975
基因名称 FAM136A
Gene ID (NCBI) 84908
RRIDAB_3671715
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96C01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM136A is an evolutively conserved nuclear gene encoding a mitochondrial protein whose specific function is unknown, but which is commonly found in neurosensory epithelial cells, where it plays a role in the electron transport chain of respiration (PMID: 37461313). FAM136A, with a molecular mass of 16-kDa, is normally expressed in the cytoplasm and has been linked to familial Meniere's disease.

实验方案

Product Specific Protocols
WB protocol for FAM136A antibody 84150-2-RRDownload protocol
IHC protocol for FAM136A antibody 84150-2-RRDownload protocol
IF protocol for FAM136A antibody 84150-2-RRDownload protocol
FC protocol for FAM136A antibody 84150-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols