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验证数据展示

  • WB analysis using 84150-2-RR (same clone as 84150-2-PBS)

    WB analysis using 84150-2-RR (same clone as 84150-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84150-2-RR (FAM136A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84150-2-RR (same clone as 84150-2-PBS)

    IHC staining of mouse testis using 84150-2-RR (same clone as 84150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84150-2-RR (same clone as 84150-2-PBS)

    IHC staining of mouse testis using 84150-2-RR (same clone as 84150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84150-2-RR (FAM136A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84150-2-RR (same clone as 84150-2-PBS)

    IF Staining of HeLa using 84150-2-RR (same clone as 84150-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FAM136A antibody (84150-2-RR, Clone: 241433D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84150-2-RR (same clone as 84150-2-PBS)

    FC experiment of U2OS using 84150-2-RR (same clone as 84150-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug FAM136A Recombinant antibody (84150-2-RR, Clone:241433D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84150-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01066-3

    Cytometric bead array standard curve of MP01066-3

    Cytometric bead array standard curve of MP01066-3, FAM136A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84150-2-PBS. Detection antibody: 84150-5-PBS. Standard: Ag34058. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84150-2-RR

    Affinity and Kinetic Characterization of 84150-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84150-2-RR against Human FAM136A were performed. The affinity constant is below 1 pM.

FAM136A Recombinant antibody, PBS Only (Capture)

FAM136A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84150-2-PBS

产品说明书

CloneNo. 241433D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

241433D12, Protein FAM136A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84150-2-PBS targets FAM136A as part of a matched antibody pair:

MP01066-3: 84150-2-PBS capture and 84150-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FAM136A fusion protein Ag34058 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 family with sequence similarity 136, member A
别名 241433D12, Protein FAM136A
观测分子量12 kDa
GenBank蛋白编号BC014975
基因名称 FAM136A
Gene ID (NCBI) 84908
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96C01
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAM136A is an evolutively conserved nuclear gene encoding a mitochondrial protein whose specific function is unknown, but which is commonly found in neurosensory epithelial cells, where it plays a role in the electron transport chain of respiration (PMID: 37461313). FAM136A, with a molecular mass of 16-kDa, is normally expressed in the cytoplasm and has been linked to familial Meniere's disease.