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验证数据展示

  • IF Staining of mouse small intestine using CL488-68227

    IF Staining of mouse small intestine using CL488-68227

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 FABP1 antibody (CL488-68227, Clone: 1B6C8 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-68227

    FC experiment of HepG2 using CL488-68227

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated FABP1 Monoclonal antibody (CL488-68227, Clone:1B6C8)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated FABP1 Monoclonal antibody

FABP1 Monoclonal Antibody for IF-P
Cat No. CL488-68227

产品说明书

CloneNo. 1B6C8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF-P

别名

liver FABP, 1B6C8, FABPL, Fatty acid binding protein 1, Fatty acid-binding protein 1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse small intestine tissue
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68227-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68227 targets FABP1 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag32836

Product name: Recombinant human FABP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-127 aa of BC032801

Sequence: MSFSGKYQLQSQENFEAFMKAIGLPEELIQKGKDIKGVSEIVQNGKHFKFTITAGSKVIQNEFTVGEECELETMTGEKVKTVVQLEGDNKLVTTFKNIKSVTELNGDIITNTMTLGDIVFKRISKRI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fatty acid binding protein 1, liver
别名 liver FABP, 1B6C8, FABPL, Fatty acid binding protein 1, Fatty acid-binding protein 1
计算分子量 127 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC032801
基因名称 FABP1
Gene ID (NCBI) 2168
RRIDAB_3084448
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP07148
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FABP1 antibody CL488-68227Download protocol
IF protocol for CL Plus 488 FABP1 antibody CL488-68227Download protocol
Standard Protocols
Click here to view our Standard Protocols
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