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验证数据展示

  • WB analysis using 68227-1-Ig

    WB analysis using 68227-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig liver using 68227-1-Ig

    WB analysis of pig liver using 68227-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 68227-1-Ig

    IHC staining of mouse liver using 68227-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68227-1-Ig (FABP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 68227-1-Ig

    IHC staining of mouse liver using 68227-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68227-1-Ig (FABP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 68227-1-Ig

    IF Staining of mouse brain using 68227-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using FABP1 antibody (68227-1-Ig, Clone: 1B6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 68227-1-Ig

    IF Staining of mouse liver using 68227-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using FABP1 antibody (68227-1-Ig, Clone: 1B6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 68227-1-Ig

    FC experiment of HepG2 using 68227-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug FABP1 Monoclonal antibody (68227-1-Ig, Clone:1B6C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

FABP1 Monoclonal antibody

FABP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 68227-1-Ig

产品说明书

CloneNo. 1B6C8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

liver FABP, 1B6C8, FABPL, Fatty acid binding protein 1, Fatty acid-binding protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inrat liver tissue, pig liver tissue, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue, mouse brain tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68227-1-Ig targets FABP1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag32836

Product name: Recombinant human FABP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-127 aa of BC032801

Sequence: MSFSGKYQLQSQENFEAFMKAIGLPEELIQKGKDIKGVSEIVQNGKHFKFTITAGSKVIQNEFTVGEECELETMTGEKVKTVVQLEGDNKLVTTFKNIKSVTELNGDIITNTMTLGDIVFKRISKRI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fatty acid binding protein 1, liver
别名 liver FABP, 1B6C8, FABPL, Fatty acid binding protein 1, Fatty acid-binding protein 1
计算分子量 127 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC032801
基因名称 FABP1
Gene ID (NCBI) 2168
RRIDAB_2935315
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP07148
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).

实验方案

Product Specific Protocols
IF protocol for FABP1 antibody 68227-1-IgDownload protocol
IHC protocol for FABP1 antibody 68227-1-IgDownload protocol
WB protocol for FABP1 antibody 68227-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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