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验证数据展示

  • WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

    WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

    WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

    FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug FABP1 Monoclonal antibody (68227-1-Ig, Clone:1B6C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

FABP1 Monoclonal antibody, PBS Only

FABP1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68227-1-PBS

产品说明书

CloneNo. 1B6C8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, FC (Intra), Indirect ELISA

别名

liver FABP, L-FABP, Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Fatty acid binding protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68227-1-PBS targets FABP1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 FABP1 fusion protein Ag32836 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fatty acid binding protein 1, liver
别名 liver FABP, L-FABP, Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Fatty acid binding protein 1
计算分子量 127 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC032801
基因名称 FABP1
Gene ID (NCBI) 2168
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP07148
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).