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验证数据展示

  • WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

    WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

    WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 68227-1-Ig (same clone as 68227-1-PBS)

    IF Staining of mouse brain using 68227-1-Ig (same clone as 68227-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using FABP1 antibody (68227-1-Ig, Clone: 1B6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 68227-1-Ig (same clone as 68227-1-PBS)

    IF Staining of mouse liver using 68227-1-Ig (same clone as 68227-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using FABP1 antibody (68227-1-Ig, Clone: 1B6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

    FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug FABP1 Monoclonal antibody (68227-1-Ig, Clone:1B6C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

FABP1 Monoclonal antibody, PBS Only

FABP1 Monoclonal Antibody for WB, IF-P, FC (Intra), Indirect ELISA
Cat No. 68227-1-PBS

产品说明书

CloneNo. 1B6C8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, FC (Intra), Indirect ELISA

别名

liver FABP, 1B6C8, FABPL, Fatty acid binding protein 1, Fatty acid-binding protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68227-1-PBS targets FABP1 in WB, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 FABP1 fusion protein Ag32836 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fatty acid binding protein 1, liver
别名 liver FABP, 1B6C8, FABPL, Fatty acid binding protein 1, Fatty acid-binding protein 1
计算分子量 127 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC032801
基因名称 FABP1
Gene ID (NCBI) 2168
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP07148
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).