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验证数据展示

  • WB analysis of Y79 using 86046-2-RR

    WB analysis of Y79 using 86046-2-RR

    Y79 cells were subjected to SDS PAGE followed by western blot with 86046-2-RR (EYA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of IMR-32 using 86046-2-RR

    WB analysis of IMR-32 using 86046-2-RR

    IMR-32 cells were subjected to SDS PAGE followed by western blot with 86046-2-RR (EYA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

EYA2 Recombinant monoclonal antibody

EYA2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86046-2-RR

产品说明书

CloneNo. 250437D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EAB1, EC:3.1.3.48

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inIMR-32 cells, Y79 cells
Planning an IHC experiment? We recommend our IHCeasy EYA2 Ready-To-Use IHC Kit. EYA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86046-2-RR targets EYA2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29403

Product name: Recombinant human EYA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 241-583 aa of BC013882

Sequence: LSYGSSFSTSPTGQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGKESCFERIMQRFGRKAVYVVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eyes absent homolog 2 (Drosophila)
别名 EAB1, EC:3.1.3.48
计算分子量 64 kDa
观测分子量60 kDa
GenBank蛋白编号BC013882
基因名称 EYA2
Gene ID (NCBI) 2139
RRIDAB_3744576
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00167
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. This recombinant antibody can not detect the modified isform with the molecular weight of 70 kda, compared with 11314-1-AP.

实验方案

Product Specific Protocols
WB protocol for EYA2 antibody 86046-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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