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验证数据展示

  • WB analysis of Y79 using 86046-2-RR (same clone as 86046-2-PBS)

    WB analysis of Y79 using 86046-2-RR (same clone as 86046-2-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 86046-2-RR (EYA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86046-2-PBS in a different storage buffer formulation.

  • WB analysis of IMR-32 using 86046-2-RR (same clone as 86046-2-PBS)

    WB analysis of IMR-32 using 86046-2-RR (same clone as 86046-2-PBS)

    IMR-32 cells were subjected to SDS PAGE followed by western blot with 86046-2-RR (EYA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86046-2-PBS in a different storage buffer formulation.

EYA2 Recombinant monoclonal antibody, PBS Only

EYA2 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86046-2-PBS

产品说明书

CloneNo. 250437D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

EAB1, EC:3.1.3.48

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EYA2 Ready-To-Use IHC Kit. EYA2 primary antibody included.

产品信息

86046-2-PBS targets EYA2 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29403

Product name: Recombinant human EYA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 241-583 aa of BC013882

Sequence: LSYGSSFSTSPTGQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGKESCFERIMQRFGRKAVYVVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eyes absent homolog 2 (Drosophila)
别名 EAB1, EC:3.1.3.48
计算分子量 64 kDa
观测分子量60 kDa
GenBank蛋白编号BC013882
基因名称 EYA2
Gene ID (NCBI) 2139
RRIDAB_3744576
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00167
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. This recombinant antibody can not detect the modified isform with the molecular weight of 70 kda, compared with 11314-1-AP.

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