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验证数据展示

  • IF Staining of Caco-2 using CL594-66818

    IF Staining of Caco-2 using CL594-66818

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite®594 ESRRB antibody (CL594-66818, Clone: 3E10C1 ) at dilution of 1:600.

  • FC experiment of Caco-2 using CL594-66818

    FC experiment of Caco-2 using CL594-66818

    1x10^6 Caco-2 cells were intracellularly stained with 0.4 ug Coralite®594 ESRRB Monoclonal Antibody (CL594-66818, Clone:3E10C1)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated ESRRB Monoclonal antibody

ESRRB Monoclonal Antibody for IF/ICC
Cat No. CL594-66818

产品说明书

CloneNo. 3E10C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

ERR-beta, ERRbeta, ERRB2, ERRb, ERR beta-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inCaco-2 cells
Positive FC (Intra) detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy ESRRB Ready-To-Use IHC Kit. ESRRB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66818-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66818 targets ESRRB in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 ESRRB fusion protein Ag18626 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 estrogen-related receptor beta
别名 ERR-beta, ERRbeta, ERRB2, ERRb, ERR beta-2
计算分子量 508 aa, 56 kDa
观测分子量 47 kDa
GenBank蛋白编号BC131517
基因名称 ESRRB
Gene ID (NCBI) 2103
RRIDAB_3673508
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO95718
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 ESRRB antibody CL594-66818Download protocol
Standard Protocols
Click here to view our Standard Protocols