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验证数据展示

  • FC experiment of MCF-7 using CL488-60197

    FC experiment of MCF-7 using CL488-60197

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ESR2 (CL488-60197, Clone:5D2C9) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis using CL488-60197

    WB analysis using CL488-60197

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-60197 (ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ESR2 Monoclonal antibody

ESR2 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-60197

产品说明书

CloneNo. 5D2C9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, FC (Intra)

别名

ESTRB, ERβ, ER-beta, Erb, ER BETA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, SW 1990 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 60197-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60197 targets ESR2 in WB, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 ESR2 fusion protein Ag5103 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 estrogen receptor 2 (ER beta)
别名 ESTRB, ERβ, ER-beta, Erb, ER BETA
计算分子量 59 kDa
观测分子量 50 kDa
GenBank蛋白编号BC024181
基因名称 ESR2
Gene ID (NCBI) 2100
RRIDAB_3084142
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92731
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa (PMID: 14766008).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 ESR2 antibody CL488-60197Download protocol
Standard Protocols
Click here to view our Standard Protocols