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ESR2 Monoclonal antibody

ESR2 Monoclonal Antibody for WB, ELISA
Cat No. 60197-1-Ig

产品说明书

CloneNo. 5D2C9

宿主/亚型

Mouse / IgG1

种属反应性

human and More (1)

应用

WB, ELISA and More (2)

ER BETA, Erb, ERβ, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF-7 cells, human brain tissue, SW 1990 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60197-1-Ig targets ESR2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 ESR2 fusion protein Ag5103 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 estrogen receptor 2 (ER beta)
别名 ER BETA, Erb, ERβ, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2
计算分子量 59 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC024181
基因名称 ESR2
Gene ID (NCBI) 2100
RRIDAB_10897162
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ92731
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa (PMID: 14766008).

实验方案

Product Specific Protocols
WB protocol for ESR2 antibody 60197-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Environ Sci (China)

Environmental microcystin exposure triggers the poor prognosis of prostate cancer: Evidence from case-control, animal, and in vitro studies

Authors - Chun Pan
mouseWB

Environ Pollut

Betulinic acid alleviates zearalenone-induced uterine injury in mice

Authors - Chenglin Yang
humanWB

Biochim Biophys Acta Mol Cell Res

IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5

Authors - Junfeng Du
mouseWB,IHC,IF

Inflammation

High Dose of Estrogen Protects the Lungs from Ischemia-Reperfusion Injury by Downregulating the Angiotensin II Signaling Pathway

Authors - Peng Dai
mouseWB

Biofactors

Propofol exerts neuroprotective functions by down-regulating microRNA-19a in glutamic acid-induced PC12 cells.

Authors - Shashuang Yu
humanWB

PeerJ

The modulatory properties of Astragalus membranaceus treatment on endometrial cancer: an integrated pharmacological method.

Authors - Qianqian Zhang
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