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验证数据展示

  • WB analysis of HUVEC using 12355-1-AP (same clone as 12355-1-PBS)

    WB analysis of HUVEC using 12355-1-AP (same clone as 12355-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 12355-1-AP (ESM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12355-1-PBS in a different storage buffer formulation.

  • IP experiment of HUVEC using 12355-1-AP (same clone as 12355-1-PBS)

    IP experiment of HUVEC using 12355-1-AP (same clone as 12355-1-PBS)

    IP result of anti-ESM1 (IP:12355-1-AP, 4ug; Detection:12355-1-AP 1:300) with HUVEC cells lysate 640 ug. This data was developed using the same antibody clone with 12355-1-PBS in a different storage buffer formulation.

ESM1 Polyclonal antibody, PBS Only

ESM1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12355-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

endocan, endothelial cell molecule 1, Endothelial cell specific molecule 1, Endothelial cell-specific molecule 1, ESM-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12355-1-PBS targets ESM1 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3019

Product name: Recombinant human ESM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-184 aa of BC011989

Sequence: SNNYAVDCPQHCDSSECKSSPRCERTVLDDCGCCRVCAAGRGETCYRTVSGMDGMKCGPGLRCQPSNGEDPFGEEFGICKDCPYGTFGMDCRETCNCQSGICDRGTGKCLKFPFFQYSVTKSSNRFVSLTEHDMASGDGNIVREEVVKENAAGSPVMRKWLNPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endothelial cell-specific molecule 1
别名 endocan, endothelial cell molecule 1, Endothelial cell specific molecule 1, Endothelial cell-specific molecule 1, ESM-1
计算分子量 184 aa, 20 kDa
观测分子量14 kDa
GenBank蛋白编号BC011989
基因名称 ESM1
Gene ID (NCBI) 11082
RRIDAB_3669137
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ30
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ESM1 is a secreted protein mainly expressed in the endothelial cells. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue was restricted to lung.

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