ESM1 Polyclonal antibody
ESM1 Polyclonal Antibody for IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IP detected in | HUVEC cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12355-1-AP targets ESM1 in IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | IP, ELISA |
| Tested Reactivity | human |
| Immunogen | ESM1 fusion protein Ag3019 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | endothelial cell-specific molecule 1 |
| Synonyms | endocan, endothelial cell molecule 1, ESM 1, ESM1 |
| Calculated Molecular Weight | 184 aa, 20 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC011989 |
| Gene Symbol | ESM1 |
| Gene ID (NCBI) | 11082 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQ30 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ESM1 is a secreted protein mainly expressed in the endothelial cells. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue was restricted to lung.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ESM1 antibody 12355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |