验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, HUVEC cells, COLO 320 cells, HepG2 cells, MOLT-4 cells, HSC-T6 cells, RAW 264.7 cells |
Planning an IHC experiment? We recommend our IHCeasy ERK1/2 Ready-To-Use IHC Kit. ERK1/2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60929-1-Ig targets ERK1/2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase 1 |
| 别名 | ERK2, MAPK1, EC:2.7.11.24, ERK, ERK 2 |
| 计算分子量 | 360 aa, 41 kDa |
| 观测分子量 | 38-44 kDa |
| GenBank蛋白编号 | BC017832 |
| 基因名称 | ERK2 |
| Gene ID (NCBI) | 5594 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P28482 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERK1 and ERK2 belong to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalyzed the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with a molecular mass of 38-44 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERK1/2 antibody 60929-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
