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验证数据展示

  • WB analysis of L02 using 84344-2-RR

    WB analysis of L02 using 84344-2-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 84344-2-RR (EPPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 84344-2-RR

    FC experiment of MCF-7 using 84344-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug EPPK1 Recombinant antibody (84344-2-RR, Clone:241633B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 84344-2-RR

    IF Staining of HeLa using 84344-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EPPK1 antibody (84344-2-RR, Clone: 241633B5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84344-2-RR

    Affinity and Kinetic Characterization of 84344-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84344-2-RR against Human EPPK1 were performed. The affinity constant is 1.61 nM.

EPPK1 Recombinant antibody

EPPK1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84344-2-RR

产品说明书

CloneNo. 241633B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

EPPK, 241633B5, EPIPL, EPIPL1, Epiplakin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84344-2-RR targets EPPK1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 EPPK1 fusion protein Ag35749 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 epiplakin 1
别名 EPPK, 241633B5, EPIPL, EPIPL1, Epiplakin
计算分子量555 kDa
观测分子量500-600 kDa
GenBank蛋白编号NM_031308.3
基因名称 EPPK1
Gene ID (NCBI) 83481
RRIDAB_3671884
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP58107
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epplakin 1 (EPPK1), belonging to the plakin family, is involved in connecting intermediate filaments and regulating their reorganization in stress response. Studies have shown that EPPK1 is involved in the progression of various cancers, such as liver cancer, cervical cancer, and bladder urothelial carcinoma.

实验方案

Product Specific Protocols
WB protocol for EPPK1 antibody 84344-2-RRDownload protocol
IF protocol for EPPK1 antibody 84344-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols