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CoraLite® Plus 488-conjugated EPHA2 Monoclonal antibody
EPHA2 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A9C3
验证数据展示
经过测试的应用
| Positive FC detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 66736-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66736 targets EPHA2 in FC (Intra) applications and shows reactivity with Human samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | Human |
| Immunogen | EPHA2 fusion protein Ag22566 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | EPH receptor A2 |
| Synonyms | ECK, EPH receptor A2, EPHA2, Ephrin type A receptor 2, Epithelial cell kinase |
| Calculated Molecular Weight | 976 aa, 108 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC037166 |
| Gene Symbol | EPHA2 |
| Gene ID (NCBI) | 1969 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P29317 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ephrin type-A receptor 2 (EPHA2), belongs to the receptor tyrosine kinases (RTKs) family, that binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (PMID: 11114742). The "reverse signaling" function, whereby the cytoplasmic domain becomes tyrosine phosphorylated, allows interactions with other proteins that may activate signaling pathways in the ligand-expressing cells.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 EPHA2 antibody CL488-66736 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
