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- KD/KO Validated
EPHA2 Monoclonal antibody
EPHA2 Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
FC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A9C3
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, BxPC-3 cells, MCF-7 cells, HepG2 cells |
| Positive FC detected in | Hela cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) | FC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
产品信息
66736-1-Ig targets EPHA2 in WB, FC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | FC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | EPHA2 fusion protein Ag22566 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | EPH receptor A2 |
| Synonyms | ECK, EPH receptor A2, EPHA2, Ephrin type A receptor 2, Epithelial cell kinase |
| Calculated Molecular Weight | 976 aa, 108 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC037166 |
| Gene Symbol | EPHA2 |
| Gene ID (NCBI) | 1969 |
| RRID | AB_2882086 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P29317 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPHA2 antibody 66736-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids EFNA4 promotes cell proliferation and tumor metastasis in hepatocellular carcinoma through a PIK3R2/GSK3β/β-catenin positive feedback loop. | ||
J Oncol EphA2 Promotes the Development of Cervical Cancer through the CXCL11/PD-L1 Pathway
| ||
J Alzheimers Dis Metabolic Reprogramming of Microglia Enhances Proinflammatory Cytokine Release through EphA2/p38 MAPK Pathway in Alzheimer's Disease
| ||
Chem Biol Interact 2-Methoxy-5((3,4,5-trimethosyphenyl)seleninyl) phenol (SQ) inhibits cancer cell metastasis behavior of TNBC via suppressing EMT and VEGF. | ||
Arch Biochem Biophys EphA2 inhibits SRA01/04 cells apoptosis by suppressing autophagy via activating PI3K/Akt/mTOR pathway.
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