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验证数据展示

  • IF Staining of HepG2 using 66736-1-Ig (same clone as 66736-1-PBS)

    IF Staining of HepG2 using 66736-1-Ig (same clone as 66736-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPHA2 antibody (66736-1-Ig, Clone: 1A9C3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

  • WB analysis using 66736-1-Ig (same clone as 66736-1-PBS)

    WB analysis using 66736-1-Ig (same clone as 66736-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66736-1-Ig (EPHA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EPHA2 Monoclonal antibody, PBS Only

EPHA2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66736-1-PBS

产品说明书

CloneNo. 1A9C3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

1A9C3, EC:2.7.10.1, ECK, EPH receptor A2, Ephrin type A receptor 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66736-1-PBS targets EPHA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22566

Product name: Recombinant human EPHA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 271-535 aa of BC037166

Sequence: DACQACSPGFFKFEASESPCLECPEHTLPSPEGATSCECEEGFFRAPQDPASMPCTRPPSAPHYLTAVGMGAKVELRWTPPQDSGGREDIVYSVTCEQCWPESGECGPCEASVRYSEPPHGLTRTSVTVSDLEPHMNYTFTVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEGRSTTSLSVSWSIPPPQQSRVWKYEVTYRKKGDSNSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSPEGSGNL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 EPH receptor A2
别名 1A9C3, EC:2.7.10.1, ECK, EPH receptor A2, Ephrin type A receptor 2
计算分子量 976 aa, 108 kDa
观测分子量 130 kDa
GenBank蛋白编号BC037166
基因名称 EPHA2
Gene ID (NCBI) 1969
RRIDAB_2882086
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP29317
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).

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