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验证数据展示

  • WB analysis using 87904-1-RR (same clone as 87904-1-PBS)

    WB analysis using 87904-1-RR (same clone as 87904-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87904-1-RR (EPHA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87904-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 87904-1-RR (same clone as 87904-1-PBS)

    IF Staining of NIH/3T3 using 87904-1-RR (same clone as 87904-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using EPHA2 antibody (87904-1-RR, Clone: 253572H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87904-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03249-1

    Cytometric bead array sample test of MP03249-1

    The mean EPHA2 concentration was determined to be 59.7 ng/mL in mouse lung tissue extract based on a 5.0 mg/mL extract load, 33.8 ng/mL in mouse spleen tissue extract based on a 7.6 mg/mL extract load, 4.6 ng/mL in mouse heart tissue extract based on a 5.4 mg/mL extract load.

  • Cytometric bead array standard curve of MP03249-1

    Cytometric bead array standard curve of MP03249-1

    Cytometric bead array standard curve of MP03249-1, MOUSE EPHA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87904-2-PBS. Detection antibody: 87904-1-PBS. Standard: Eg8778. Range: 0.312-40 ng/mL.

  • Affinity of 87904-1-RR

    Affinity of 87904-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87904-1-RR against Mouse EPHA2 were performed. The affinity constant is below 1 pM.

Mouse EPHA2 Recombinant monoclonal antibody, PBS Only (Detector)

EPHA2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 87904-1-PBS

产品说明书

CloneNo. 253572H2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

EC:2.7.10.1, Eck, Ephrin type-A receptor 2, Epithelial cell kinase, Myk2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87904-1-PBS targets EPHA2 as part of a matched antibody pair:

MP03249-1: 87904-2-PBS capture and 87904-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg8778

Product name: Recombinant mouse Epha2 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 26-538 aa of NM_010139.3

Sequence: KEVVLLDFAAMKGELGWLTHPYGKGWDLMQNIMDDMPIYMYSVCNVVSGDQDNWLRTNWVYREEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDVDYGTNFQKRQFTKIDTIAPDEITVSSDFEARNVKLNVEERMVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPEMLQSLARFPETIAVAVSDTQPLATVAGTCVDHAVVPYGGEGPLMHCTVDGEWLVPIGQCLCQEGYEKVEDACRACSPGFFKSEASESPCLECPEHTLPSTEGATSCQCEEGYFRAPEDPLSMSCTRPPSAPNYLTAIGMGAKVELRWTAPKDTGGRQDIVYSVTCEQCWPESGECGPCEASVRYSEPPHALTRTSVTVSDLEPHMNYTFAVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEDRSTTSLSVTWSIPVSQQSRVWKYEVTYRKKGDANSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSTEGSANMAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Eph receptor A2
别名 EC:2.7.10.1, Eck, Ephrin type-A receptor 2, Epithelial cell kinase, Myk2
计算分子量109 kDa
观测分子量130 kDa
GenBank蛋白编号NM_010139.3
基因名称 Epha2
Gene ID (NCBI) 13836
RRIDAB_3745819
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ03145
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPH receptor A2 (EPHA2) is a receptor tyrosine kinase (RTK) of the Eph receptor family that primarily binds glycosylphosphatidylinositol (GPI)-anchored ephrin-A ligands, especially ephrin-A1 (EFNA1), to mediate contact-dependent cell-cell signaling. In mice, Epha2 is broadly expressed in epithelial, endothelial, and selected stromal compartments, where it regulates cell adhesion, polarity, migration, proliferation, barrier organization, and tissue architecture.

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