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验证数据展示

  • WB analysis using 87904-1-RR

    WB analysis using 87904-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87904-1-RR (EPHA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 87904-1-RR

    IF Staining of NIH/3T3 using 87904-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using EPHA2 antibody (87904-1-RR, Clone: 253572H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87904-1-RR

    Affinity of 87904-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87904-1-RR against Mouse EPHA2 were performed. The affinity constant is below 1 pM.

EPHA2 Recombinant monoclonal antibody

EPHA2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87904-1-RR

产品说明书

CloneNo. 253572H2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

EC:2.7.10.1, Eck, Ephrin type-A receptor 2, Epithelial cell kinase, Myk2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL-929 cells, NIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87904-1-RR targets EPHA2 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg8778

Product name: Recombinant mouse Epha2 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 26-538 aa of NM_010139.3

Sequence: KEVVLLDFAAMKGELGWLTHPYGKGWDLMQNIMDDMPIYMYSVCNVVSGDQDNWLRTNWVYREEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDVDYGTNFQKRQFTKIDTIAPDEITVSSDFEARNVKLNVEERMVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPEMLQSLARFPETIAVAVSDTQPLATVAGTCVDHAVVPYGGEGPLMHCTVDGEWLVPIGQCLCQEGYEKVEDACRACSPGFFKSEASESPCLECPEHTLPSTEGATSCQCEEGYFRAPEDPLSMSCTRPPSAPNYLTAIGMGAKVELRWTAPKDTGGRQDIVYSVTCEQCWPESGECGPCEASVRYSEPPHALTRTSVTVSDLEPHMNYTFAVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEDRSTTSLSVTWSIPVSQQSRVWKYEVTYRKKGDANSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSTEGSANMAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Eph receptor A2
别名 EC:2.7.10.1, Eck, Ephrin type-A receptor 2, Epithelial cell kinase, Myk2
计算分子量109 kDa
观测分子量130 kDa
GenBank蛋白编号NM_010139.3
基因名称 Epha2
Gene ID (NCBI) 13836
RRIDAB_3745819
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ03145
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPH receptor A2 (EPHA2) is a receptor tyrosine kinase (RTK) of the Eph receptor family that primarily binds glycosylphosphatidylinositol (GPI)-anchored ephrin-A ligands, especially ephrin-A1 (EFNA1), to mediate contact-dependent cell-cell signaling. In mice, Epha2 is broadly expressed in epithelial, endothelial, and selected stromal compartments, where it regulates cell adhesion, polarity, migration, proliferation, barrier organization, and tissue architecture.

实验方案

Product Specific Protocols
IF protocol for EPHA2 antibody 87904-1-RRDownload protocol
WB protocol for EPHA2 antibody 87904-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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