ENPEP Recombinant antibody

ENPEP Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85963-1-RR

产品说明书

CloneNo. 250264B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

AP A, APA, AP-A, CD249, EAP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse kidney tissue, human kidney tissue, rat lung tissue, rat kidney tissue, mouse lung tissue, =

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85963-1-RR targets ENPEP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ENPEP fusion protein Eg2736 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutamyl aminopeptidase (aminopeptidase A)
别名 AP A, APA, AP-A, CD249, EAP
计算分子量109 kDa
观测分子量140 kDa
GenBank蛋白编号NM_001977.3
基因名称 ENPEP
Gene ID (NCBI) 2028
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07075
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.

实验方案

Product Specific Protocols
WB protocol for ENPEP antibody 85963-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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