验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, human kidney tissue, rat lung tissue, rat kidney tissue, mouse lung tissue, = |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85963-1-RR targets ENPEP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ENPEP fusion protein Eg2736 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | glutamyl aminopeptidase (aminopeptidase A) |
| 别名 | AP A, APA, AP-A, CD249, EAP |
| 计算分子量 | 109 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | NM_001977.3 |
| 基因名称 | ENPEP |
| Gene ID (NCBI) | 2028 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q07075 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ENPEP antibody 85963-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |