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验证数据展示

  • WB analysis using 85963-1-RR (same clone as 85963-1-PBS)

    WB analysis using 85963-1-RR (same clone as 85963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85963-1-PBS in a different storage buffer formulation.

  • WB analysis of rat lung using 85963-1-RR (same clone as 85963-1-PBS)

    WB analysis of rat lung using 85963-1-RR (same clone as 85963-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85963-1-PBS in a different storage buffer formulation.

  • WB analysis of human kidney using 85963-1-RR (same clone as 85963-1-PBS)

    WB analysis of human kidney using 85963-1-RR (same clone as 85963-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85963-1-PBS in a different storage buffer formulation.

ENPEP Recombinant monoclonal antibody, PBS Only

ENPEP Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85963-1-PBS

产品说明书

CloneNo. 250264B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

AP A, APA, AP-A, CD249, EAP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85963-1-PBS targets ENPEP in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg2736

Product name: Recombinant Human ENPEP protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 41-957 aa of NM_001977.3

Sequence: RSCDSSGDGGPGTAPAPSHLPSSTASPSGPPAQDQDICPASEDESGQWKNFRLPDFVNPVHYDLHVKPLLEEDTYTGTVSISINLSAPTRYLWLHLRETRITRLPELKRPSGDQVQVRRCFEYKKQEYVVVEAEEELTPSSGDGLYLLTMEFAGWLNGSLVGFYRTTYTENGQVKSIVATDHEPTDARKSFPCFDEPNKKATYTISITHPKEYGALSNMPVAKEESVDDKWTRTTFEKSVPMSTYLVCFAVHQFDSVKRISNSGKPLTIYVQPEQKHTAEYAANITKSVFDYFEEYFAMNYSLPKLDKIAIPDFGTGAMENWGLITYRETNLLYDPKESASSNQQRVATVVAHELVHQWFGNIVTMDWWEDLWLNEGFASFFEFLGVNHAETDWQMRDQMLLEDVLPVQEDDSLMSSHPIIVTVTTPDEITSVFDGISYSKGSSILRMLEDWIKPENFQKGCQMYLEKYQFKNAKTSDFWAALEEASRLPVKEVMDTWTRQMGYPVLNVNGVKNITQKRFLLDPRANPSQPPSDLGYTWNIPVKWTEDNITSSVLFNRSEKEGITLNSSNPSGNAFLKINPDHIGFYRVNYEVATWDSIATALSLNHKTFSSADRASLIDDAFALARAQLLDYKVALNLTKYLKREENFLPWQRVISAVTYIISMFEDDKELYPMIEEYFQGQVKPIADSLGWNDAGDHVTKLLRSSVLGFACKMGDREALNNASSLFEQWLNGTVSLPVNLRLLVYRYGMQNSGNEISWNYTLEQYQKTSLAQEKEKLLYGLASVKNVTLLSRYLDLLKDTNLIKTQDVFTVIRYISYNSYGKNMAWNWIQLNWDYLVNRYTLNNRNLGRIVTIAEPFNTELQLWQMESFFAKYPQAGAGEKPREQVLETVKNNIEWLKQHRNTIREWFFNLLESG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutamyl aminopeptidase (aminopeptidase A)
别名 AP A, APA, AP-A, CD249, EAP
计算分子量109 kDa
观测分子量140 kDa
GenBank蛋白编号NM_001977.3
基因名称 ENPEP
Gene ID (NCBI) 2028
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ07075
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.

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