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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85963-1-RR (same clone as 85963-1-PBS)

    WB analysis using 85963-1-RR (same clone as 85963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85963-1-PBS in a different storage buffer formulation.

  • WB analysis of rat lung using 85963-1-RR (same clone as 85963-1-PBS)

    WB analysis of rat lung using 85963-1-RR (same clone as 85963-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85963-1-PBS in a different storage buffer formulation.

  • WB analysis of human kidney using 85963-1-RR (same clone as 85963-1-PBS)

    WB analysis of human kidney using 85963-1-RR (same clone as 85963-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85963-1-PBS in a different storage buffer formulation.

ENPEP Recombinant antibody, PBS Only

ENPEP Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85963-1-PBS

产品说明书

CloneNo. 250264B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

AP A, APA, AP-A, CD249, EAP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85963-1-PBS targets ENPEP in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ENPEP fusion protein Eg2736 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutamyl aminopeptidase (aminopeptidase A)
别名 AP A, APA, AP-A, CD249, EAP
计算分子量109 kDa
观测分子量140 kDa
GenBank蛋白编号NM_001977.3
基因名称 ENPEP
Gene ID (NCBI) 2028
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07075
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.