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验证数据展示

  • IF Staining of HeLa using CL488-68147

    IF Staining of HeLa using CL488-68147

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ENO3 antibody (CL488-68147, Clone: 2C12G3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ENO3 Monoclonal antibody

ENO3 Monoclonal Antibody for IF/ICC
Cat No. CL488-68147

产品说明书

CloneNo. 2C12G3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig

应用

IF/ICC

别名

Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ENO3 Ready-To-Use IHC Kit. ENO3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68147-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68147 targets ENO3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 ENO3 fusion protein Ag9642 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 enolase 3 (beta, muscle)
别名 Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase
计算分子量 434 aa, 47 kDa
观测分子量42-47 kDa
GenBank蛋白编号BC017249
基因名称 ENO3
Gene ID (NCBI) 2027
RRIDAB_3084418
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13929
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ENO3 antibody CL488-68147Download protocol
Standard Protocols
Click here to view our Standard Protocols