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验证数据展示

  • WB analysis using 68147-1-Ig

    WB analysis using 68147-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68147-1-Ig (ENO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68147-1-Ig

    IF Staining of HeLa using 68147-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ENO3 antibody (68147-1-Ig, Clone: 2C12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ENO3 Monoclonal antibody

ENO3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68147-1-Ig

产品说明书

CloneNo. 2C12G3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IF/ICC, ELISA

别名

Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig skeletal muscle tissue, rabbit skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ENO3 Ready-To-Use IHC Kit. ENO3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68147-1-Ig targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 ENO3 fusion protein Ag9642 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 enolase 3 (beta, muscle)
别名 Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase
计算分子量 434 aa, 47 kDa
观测分子量42-47 kDa
GenBank蛋白编号BC017249
基因名称 ENO3
Gene ID (NCBI) 2027
RRIDAB_2923671
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13929
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693)

实验方案

Product Specific Protocols
WB protocol for ENO3 antibody 68147-1-IgDownload protocol
IF protocol for ENO3 antibody 68147-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols