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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 68147-1-Ig (same clone as 68147-1-PBS)

    WB analysis using 68147-1-Ig (same clone as 68147-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68147-1-Ig (ENO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68147-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68147-1-Ig (same clone as 68147-1-PBS)

    IF Staining of HeLa using 68147-1-Ig (same clone as 68147-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ENO3 antibody (68147-1-Ig, Clone: 2C12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68147-1-PBS in a different storage buffer formulation.

ENO3 Monoclonal antibody, PBS Only

ENO3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68147-1-PBS

产品说明书

CloneNo. 2C12G3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IF/ICC, Indirect ELISA

别名

Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ENO3 Ready-To-Use IHC Kit. ENO3 primary antibody included.

产品信息

68147-1-PBS targets ENO3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 ENO3 fusion protein Ag9642 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 enolase 3 (beta, muscle)
别名 Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase
计算分子量 434 aa, 47 kDa
观测分子量42-47 kDa
GenBank蛋白编号BC017249
基因名称 ENO3
Gene ID (NCBI) 2027
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13929
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693)