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验证数据展示

  • IF Staining of HeLa using 82888-1-RR

    IF Staining of HeLa using 82888-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-1-RR, Clone: 230089B1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

  • FC experiment of HeLa using 82888-1-RR

    FC experiment of HeLa using 82888-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Emerin Recombinant antibody (82888-1-RR, Clone:230089B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human stomach cancer using 82888-1-RR

    IHC staining of human stomach cancer using 82888-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-1-RR (Emerin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Affinity and Kinetic Characterization of 82888-1-RR

    Affinity and Kinetic Characterization of 82888-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82888-1-RR against Human Emerin were performed. The affinity constant is 5.36 nM.

Emerin Recombinant antibody

Emerin Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82888-1-RR

产品说明书

CloneNo. 230089B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

EMD, STA, LEMD5, EDMD, 230089B1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82888-1-RR targets Emerin in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Emerin fusion protein Ag0236 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 emerin
别名 EMD, STA, LEMD5, EDMD, 230089B1
计算分子量 34 kDa
GenBank蛋白编号BC000738
基因名称 EMD
Gene ID (NCBI) 2010
RRIDAB_3670608
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50402
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

实验方案

Product Specific Protocols
IHC protocol for Emerin antibody 82888-1-RRDownload protocol
IF protocol for Emerin antibody 82888-1-RRDownload protocol
FC protocol for Emerin antibody 82888-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols