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验证数据展示

  • IF Staining of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    IF Staining of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-1-RR, Clone: 230089B1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    FC experiment of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Emerin Recombinant antibody (82888-1-RR, Clone:230089B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 82888-1-RR (same clone as 82888-1-PBS)

    IHC staining of human stomach cancer using 82888-1-RR (same clone as 82888-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-1-RR (Emerin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82888-1-RR

    Affinity and Kinetic Characterization of 82888-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82888-1-RR against Human Emerin were performed. The affinity constant is 5.36 nM.

Emerin Recombinant antibody, PBS Only

Emerin Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82888-1-PBS

产品说明书

CloneNo. 230089B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

EMD, STA, LEMD5, EDMD, 230089B1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82888-1-PBS targets Emerin in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Emerin fusion protein Ag0236 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 emerin
别名 EMD, STA, LEMD5, EDMD, 230089B1
计算分子量 34 kDa
GenBank蛋白编号BC000738
基因名称 EMD
Gene ID (NCBI) 2010
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50402
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.