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EIF3L Monoclonal antibody

EIF3L Monoclonal Antibody for WB,IF/ICC,IF-P,FC,IHC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB,IF/ICC,IF-P,FC,IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1B11A10

Cat No : 67719-1-Ig

Print datasheet

Synonyms

EIF3EIP, EIF3L, EIF3S11, EIF3S6IP, HSPC021, HSPC025, MSTP005

验证数据展示

  • WB analysis using 67719-1-Ig

    WB analysis using 67719-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67719-1-Ig (EIF3L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 67719-1-Ig

    IHC staining of mouse pancreas using 67719-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67719-1-Ig (EIF3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 67719-1-Ig

    IHC staining of mouse pancreas using 67719-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67719-1-Ig (EIF3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 67719-1-Ig

    IF Staining of mouse pancreas using 67719-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse pancreas using 67719-1-Ig

    IF Staining of mouse pancreas using 67719-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 67719-1-Ig

    FC experiment of HeLa using 67719-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EIF3L (67719-1-Ig, Clone:1B11A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells, PC-12 cells, 4T1 cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue, HeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67719-1-Ig targets EIF3L in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IF/ICC,IF-P,FC,IHC,ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen EIF3L fusion protein Ag26680 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit L
Synonyms EIF3EIP, EIF3L, EIF3S11, EIF3S6IP, HSPC021, HSPC025, MSTP005
Calculated Molecular Weight 67 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC007510
Gene Symbol EIF3L
Gene ID (NCBI) 51386
RRIDAB_2882907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y262
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF3L antibody 67719-1-IgDownload protocol
IHC protocol for EIF3L antibody 67719-1-IgDownload protocol
IF protocol for EIF3L antibody 67719-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols