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CoraLite® Plus 647-conjugated EIF3L Monoclonal antibody

EIF3L Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1B11A10

Cat No : CL647-67719

Print datasheet

Synonyms

EIF3EIP, EIF3L, EIF3S11, EIF3S6IP, HSPC021, HSPC025, MSTP005

验证数据展示

  • FC experiment of HeLa using CL647-67719

    FC experiment of HeLa using CL647-67719

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human EIF3L (CL647-67719, Clone:1B11A10) (red), or 0.8 ug CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67719-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67719 targets EIF3L in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, Mouse, Rat
Immunogen EIF3L fusion protein Ag26680 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit L
Synonyms EIF3EIP, EIF3L, EIF3S11, EIF3S6IP, HSPC021, HSPC025, MSTP005
Calculated Molecular Weight 67 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC007510
Gene Symbol EIF3L
Gene ID (NCBI) 51386
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y262
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 EIF3L antibody CL647-67719Download protocol
Standard Protocols
Click here to view our Standard Protocols