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验证数据展示

  • IF Staining of HeLa using CL488-67674

    IF Staining of HeLa using CL488-67674

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 EIF2AK1 antibody (CL488-67674, Clone: 2H1F3 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67674

    FC experiment of HepG2 using CL488-67674

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated EIF2AK1 Monoclonal antibody (CL488-67674, Clone:2H1F3)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated EIF2AK1 Monoclonal antibody

EIF2AK1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67674

产品说明书

CloneNo. 2H1F3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

2H1F3, EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF2AK1 Ready-To-Use IHC Kit. EIF2AK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67674-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67674 targets EIF2AK1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag16970

Product name: Recombinant human EIF2AK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 281-630 aa of BC006524

Sequence: FAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 1
别名 2H1F3, EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor
计算分子量 630 aa, 71 kDa
观测分子量71 kDa
GenBank蛋白编号BC006524
基因名称 EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_3672958
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BQI3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 EIF2AK1 antibody CL488-67674Download protocol
IF protocol for CL Plus 488 EIF2AK1 antibody CL488-67674Download protocol
Standard Protocols
Click here to view our Standard Protocols
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