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EIF2AK1 Monoclonal antibody

EIF2AK1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H1F3

Cat No : 67674-1-Ig

Print datasheet

Synonyms

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369

验证数据展示

  • WB analysis using 67674-1-Ig

    WB analysis using 67674-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67674-1-Ig

    IHC staining of human breast cancer using 67674-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67674-1-Ig

    IHC staining of human breast cancer using 67674-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67674-1-Ig

    IF Staining of HeLa using 67674-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2AK1 antibody (67674-1-Ig, Clone: 2H1F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67674-1-Ig targets EIF2AK1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human
Immunogen EIF2AK1 fusion protein Ag16970 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Synonyms EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight71 kDa
GenBank Accession NumberBC006524
Gene Symbol EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_2882869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BQI3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

实验方案

Product Specific Protocols
WB protocol for EIF2AK1 antibody 67674-1-IgDownload protocol
IHC protocol for EIF2AK1 antibody 67674-1-IgDownload protocol
IF protocol for EIF2AK1 antibody 67674-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols