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验证数据展示

  • FC experiment of MCF-7 using CL488-66689

    FC experiment of MCF-7 using CL488-66689

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EHMT2 (CL488-66689, Clone:1A12E3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated EHMT2 Monoclonal antibody

EHMT2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66689

产品说明书

CloneNo. 1A12E3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

EHMT2/G9a, Euchromatic histone-lysine N-methyltransferase 2, EC:2.1.1.-, C6orf30, BAT8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66689-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66689 targets EHMT2 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 EHMT2 fusion protein Ag27477 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 euchromatic histone-lysine N-methyltransferase 2
别名 EHMT2/G9a, Euchromatic histone-lysine N-methyltransferase 2, EC:2.1.1.-, C6orf30, BAT8
计算分子量 1210 aa, 132 kDa
观测分子量 150 kDa and 170 kDa
GenBank蛋白编号BC018718
基因名称 EHMT2
Gene ID (NCBI) 10919
RRIDAB_3084265
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96KQ7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 EHMT2 antibody CL488-66689Download protocol
Standard Protocols
Click here to view our Standard Protocols