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验证数据展示

  • IF Staining of mouse testis using 17352-1-AP (same clone as 17352-1-PBS)

    IF Staining of mouse testis using 17352-1-AP (same clone as 17352-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EFHB antibody (17352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17352-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 17352-1-AP (same clone as 17352-1-PBS)

    IHC staining of mouse testis using 17352-1-AP (same clone as 17352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17352-1-AP (EFHB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17352-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 17352-1-AP (same clone as 17352-1-PBS)

    IF Staining of mouse testis using 17352-1-AP (same clone as 17352-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EFHB antibody (17352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17352-1-PBS in a different storage buffer formulation.

EFHB Polyclonal antibody, PBS Only

EFHB Polyclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 17352-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, Indirect ELISA

别名

CFAP21, Cilia- and flagella-associated protein 21, EF-hand domain-containing family member B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17352-1-PBS targets EFHB in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11431

Product name: Recombinant human EFHB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 319-669 aa of BC028198

Sequence: RADDFKEKFQHKLGRVLDPIAETMNVPPDCTFGACLRPEEYGVGDLIHNRLPDEYLRGKDRQRALIAAVRHHLKKVNYQKFDTLLAAFRHYDKKGDGMIDKDELQEACDQANLSLDDKLLDQLFDYCDVDNDGFINYLEFANFLNWKDKMLLKEYEERVIIKGRKPDCVNPTEANVEEPEQTLLIKPEDIVLKEAGSTEKTLRTLLRPSDKVSNYYKTTSSEINAIVGAIPSTCYPICGVPTIRSDIPAPRIRRISDRTNYGEEGSAYSLLYPTIFARKGVFERDFFKTRSKEEIAEILCNIGVKLSDEEFENVWNLASKKHHRGEVCVENIRNVLDELRHADRIKCKTLM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EF-hand domain family, member B
别名 CFAP21, Cilia- and flagella-associated protein 21, EF-hand domain-containing family member B
计算分子量669aa,76 kDa; 831aa,94 kDa
GenBank蛋白编号BC028198
基因名称 EFHB
Gene ID (NCBI) 151651
RRIDAB_3741917
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N7U6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EFHB (EF-hand domain family member B, also known as CFAP21) interacts with Orai1 in a Ca2+-dependent manner. Human EFHB is an 833-amino acid cytosolic protein with two EF-hand domains between amino acids 561-596 and 597-632 (PMID: 34439314).

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