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验证数据展示

  • IF Staining of mouse testis using 17352-1-AP

    IF Staining of mouse testis using 17352-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EFHB antibody (17352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 17352-1-AP

    IHC staining of mouse testis using 17352-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17352-1-AP (EFHB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 17352-1-AP

    IF Staining of mouse testis using 17352-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EFHB antibody (17352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EFHB Polyclonal antibody

EFHB Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 17352-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

CFAP21, Cilia- and flagella-associated protein 21, EF-hand domain-containing family member B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17352-1-AP targets EFHB in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11431

Product name: Recombinant human EFHB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 319-669 aa of BC028198

Sequence: RADDFKEKFQHKLGRVLDPIAETMNVPPDCTFGACLRPEEYGVGDLIHNRLPDEYLRGKDRQRALIAAVRHHLKKVNYQKFDTLLAAFRHYDKKGDGMIDKDELQEACDQANLSLDDKLLDQLFDYCDVDNDGFINYLEFANFLNWKDKMLLKEYEERVIIKGRKPDCVNPTEANVEEPEQTLLIKPEDIVLKEAGSTEKTLRTLLRPSDKVSNYYKTTSSEINAIVGAIPSTCYPICGVPTIRSDIPAPRIRRISDRTNYGEEGSAYSLLYPTIFARKGVFERDFFKTRSKEEIAEILCNIGVKLSDEEFENVWNLASKKHHRGEVCVENIRNVLDELRHADRIKCKTLM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EF-hand domain family, member B
别名 CFAP21, Cilia- and flagella-associated protein 21, EF-hand domain-containing family member B
计算分子量669aa,76 kDa; 831aa,94 kDa
GenBank蛋白编号BC028198
基因名称 EFHB
Gene ID (NCBI) 151651
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N7U6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EFHB (EF-hand domain family member B, also known as CFAP21) interacts with Orai1 in a Ca2+-dependent manner. Human EFHB is an 833-amino acid cytosolic protein with two EF-hand domains between amino acids 561-596 and 597-632 (PMID: 34439314).

实验方案

Product Specific Protocols
IHC protocol for EFHB antibody 17352-1-APDownload protocol
IF protocol for EFHB antibody 17352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols