验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67550 targets EEF2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag13859 Product name: Recombinant human EEF2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 509-858 aa of BC126259 Sequence: VEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation elongation factor 2 |
| 别名 | 1E4D5, EC:3.6.5.-, EF 2, EF2, EF-2 |
| 计算分子量 | 858 aa, 95 kDa |
| GenBank蛋白编号 | BC126259 |
| 基因名称 | EEF2 |
| Gene ID (NCBI) | 1938 |
| RRID | AB_2920157 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 激发激光 | Yellow-Green Laser (561 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P13639 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 EEF2 antibody CL594-67550 | Download protocol |
| IF protocol for CL594 EEF2 antibody CL594-67550 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
