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验证数据展示

  • IF Staining of HeLa using CL594-60085

    IF Staining of HeLa using CL594-60085

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 EEF1D antibody (CL594-60085, Clone: 3B1B11 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL594-60085

    FC experiment of MCF-7 using CL594-60085

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EEF1D (CL594-60085, Clone:3B1B11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated EEF1D Monoclonal antibody

EEF1D Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-60085

产品说明书

CloneNo. 3B1B11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

3B1B11, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EEF1D Ready-To-Use IHC Kit. EEF1D primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60085-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60085 targets EEF1D in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0983

Product name: Recombinant human EEF1D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 347-647 aa of BC007847

Sequence: SHRPGPRSGLSVSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
别名 3B1B11, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D
计算分子量 31 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC007847
基因名称 EEF1D
Gene ID (NCBI) 1936
RRIDAB_2883420
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP29692
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

实验方案

Product Specific Protocols
IF protocol for CL594 EEF1D antibody CL594-60085Download protocol
Standard Protocols
Click here to view our Standard Protocols
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