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验证数据展示

  • WB analysis using 81377-1-RR (same clone as 81377-1-PBS)

    WB analysis using 81377-1-RR (same clone as 81377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81377-1-RR (EEF1A1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81377-1-RR (same clone as 81377-1-PBS)

    WB analysis of HepG2 using 81377-1-RR (same clone as 81377-1-PBS)

    WB result of EEF1A1 antibody (81377-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HepG2 cells. This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81377-1-RR (same clone as 81377-1-PBS)

    IP experiment of HeLa using 81377-1-RR (same clone as 81377-1-PBS)

    IP result of anti-EEF1A1 (IP:81377-1-RR, 4ug; Detection:81377-1-RR 1:8000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 81377-1-RR (same clone as 81377-1-PBS)

    IHC staining of mouse brain using 81377-1-RR (same clone as 81377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A1 antibody included
  • IHC staining of mouse brain using 81377-1-RR (same clone as 81377-1-PBS)

    IHC staining of mouse brain using 81377-1-RR (same clone as 81377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A1 antibody included
  • IF Staining of HepG2 using 81377-1-RR (same clone as 81377-1-PBS)

    IF Staining of HepG2 using 81377-1-RR (same clone as 81377-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EEF1A1 antibody (81377-1-RR, Clone: 2B16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81377-1-RR

    Affinity and Kinetic Characterization of 81377-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81377-1-RR against Human EEF1A1 were performed. The affinity constant is 25.3 nM.

  • Featured Product
  • KD/KO验证

EEF1A1 Recombinant monoclonal antibody, PBS Only

EEF1A1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 81377-1-PBS

产品说明书

CloneNo. 2B16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EEF1A, EEF 1, EC:3.6.5.-, CCS3, CCS 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EEF1A1 Ready-To-Use IHC Kit. EEF1A1 primary antibody included.

产品信息

81377-1-PBS targets EEF1A1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1938

Product name: Recombinant human EEF1A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 144-462 aa of BC014224

Sequence: GVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation elongation factor 1 alpha 1
别名 EEF1A, EEF 1, EC:3.6.5.-, CCS3, CCS 3
计算分子量 462 aa, 50 kDa
观测分子量50 kDa
GenBank蛋白编号BC014224
基因名称 EEF1A1
Gene ID (NCBI) 1915
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP68104
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.1 It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.

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