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验证数据展示

  • WB analysis using 66735-1-Ig (same clone as 66735-1-PBS)

    WB analysis using 66735-1-Ig (same clone as 66735-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66735-1-Ig (Dystroglycan antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAG1 antibody included
  • IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAG1 antibody included
  • IF Staining of HeLa using 66735-1-Ig (same clone as 66735-1-PBS)

    IF Staining of HeLa using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Dystroglycan antibody (66735-1-Ig, Clone: 2B1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66735-1-Ig (same clone as 66735-1-PBS)

    IF Staining of A549 using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Dystroglycan antibody (66735-1-Ig, Clone: 2B1G12 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66735-1-Ig (same clone as 66735-1-PBS)

    IHC staining of human colon using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAG1 antibody included
  • Featured Product
  • KD/KO验证

Dystroglycan Monoclonal antibody, PBS Only

Dystroglycan Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66735-1-PBS

产品说明书

CloneNo. 2B1G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DAG1, DAG, Beta DG, Alpha DG Beta dystroglycan, AGRNR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DAG1 Ready-To-Use IHC Kit. DAG1 primary antibody included.

产品信息

66735-1-PBS targets Dystroglycan in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Dystroglycan fusion protein Ag27222 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dystroglycan 1 (dystrophin-associated glycoprotein 1)
别名 DAG1, DAG, Beta DG, Alpha DG Beta dystroglycan, AGRNR
计算分子量 97 kDa
观测分子量 43 kDa, 30 kDa
GenBank蛋白编号BC012740
基因名称 Dystroglycan
Gene ID (NCBI) 1605
RRIDAB_2882085
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14118
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).