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  • Featured Product
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Dystroglycan Monoclonal antibody

Dystroglycan Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B1G12

Cat No : 66735-1-Ig

Print datasheet

Synonyms

156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan

验证数据展示

  • WB analysis using 66735-1-Ig

    WB analysis using 66735-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66735-1-Ig (Dystroglycan antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 66735-1-Ig

    IHC staining of mouse heart using 66735-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DAG1 antibody included
  • IHC staining of mouse heart using 66735-1-Ig

    IHC staining of mouse heart using 66735-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DAG1 antibody included
  • IF Staining of HeLa using 66735-1-Ig

    IF Staining of HeLa using 66735-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Dystroglycan antibody (66735-1-Ig, Clone: 2B1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66735-1-Ig

    FC experiment of HeLa using 66735-1-Ig

    1X10^6 HeLa cells were stained with 0.20ug Dystroglycan antibody (66735-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

经过测试的应用

Positive WB detected inA549 cells, NCI-H1299 cells, HeLa cells, HepG2 cells, pig brain tissue, rat brain tissue, mouse brain
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DAG1 Ready-To-Use IHC Kit. DAG1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

66735-1-Ig targets Dystroglycan in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsFC
Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman
Immunogen Dystroglycan fusion protein Ag27222 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dystroglycan 1 (dystrophin-associated glycoprotein 1)
Synonyms 156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 43 kDa, 30 kDa
GenBank Accession NumberBC012740
Gene Symbol DAG1
Gene ID (NCBI) 1605
RRIDAB_2882085
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14118
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).

实验方案

Product Specific Protocols
WB protocol for Dystroglycan antibody 66735-1-IgDownload protocol
IHC protocol for Dystroglycan antibody 66735-1-IgDownload protocol
IF protocol for Dystroglycan antibody 66735-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

Proc Natl Acad Sci U S A

CACNA1S haploinsufficiency confers resistance to New World arenavirus infection.

Authors - Nicolás Sarute
View Article