验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy Desmin Ready-To-Use IHC Kit. Desmin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82748-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-82748 targets Desmin in IF-P applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag9742 Product name: Source: e coli.-derived, PGEX-4T Tag: GST Sequence: 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | desmin |
| 别名 | 1D21, DES |
| 计算分子量 | 470 aa, 54 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC032116 |
| 基因名称 | Desmin |
| Gene ID (NCBI) | 1674 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17661 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 Desmin antibody CL647-82748 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |