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验证数据展示

  • WB analysis using 82748-1-RR

    WB analysis using 82748-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82748-1-RR (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 82748-1-RR

    IHC staining of mouse skeletal muscle using 82748-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Desmin antibody included
  • IHC staining of mouse skeletal muscle using 82748-1-RR

    IHC staining of mouse skeletal muscle using 82748-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Desmin antibody included
  • IHC staining of mouse heart using 82748-1-RR

    IHC staining of mouse heart using 82748-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Desmin antibody included
  • IHC staining of mouse heart using 82748-1-RR

    IHC staining of mouse heart using 82748-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Desmin antibody included
  • Affinity and Kinetic Characterization of 82748-1-RR

    Affinity and Kinetic Characterization of 82748-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82748-1-RR against Human Desmin were performed. The affinity constant is 1.32 nM.

Desmin Recombinant antibody

Desmin Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82748-1-RR

产品说明书

CloneNo. 1D21

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

1D21, DES

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC2C12 cells, pig heart tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Desmin Ready-To-Use IHC Kit. Desmin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82748-1-RR targets Desmin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Desmin fusion protein Ag9742 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 desmin
别名 1D21, DES
计算分子量 470 aa, 54 kDa
观测分子量 53 kDa
GenBank蛋白编号BC032116
基因名称 Desmin
Gene ID (NCBI) 1674
RRIDAB_3086528
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP17661
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

实验方案

Product Specific Protocols
WB protocol for Desmin antibody 82748-1-RRDownload protocol
IHC protocol for Desmin antibody 82748-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols