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验证数据展示

  • IF Staining of HepG2 using CL488-68312

    IF Staining of HepG2 using CL488-68312

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DYNLT1 antibody (CL488-68312, Clone: 2E3D4 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated DYNLT1 Monoclonal antibody

DYNLT1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68312

产品说明书

CloneNo. 2E3D4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC

别名

T-complex testis-specific protein 1 homolog, Protein CW-1, Protein CW 1, Dynein light chain Tctex-type 1, CW 1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DYNLT1 Ready-To-Use IHC Kit. DYNLT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68312-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68312 targets DYNLT1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag33072

Product name: Recombinant human DYNLT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-113 aa of BC029412

Sequence: MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 dynein, light chain, Tctex-type 1
别名 T-complex testis-specific protein 1 homolog, Protein CW-1, Protein CW 1, Dynein light chain Tctex-type 1, CW 1
计算分子量 113 aa, 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC029412
基因名称 DYNLT1
Gene ID (NCBI) 6993
RRIDAB_3672993
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP63172
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (PMID: 19448628)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DYNLT1 antibody CL488-68312Download protocol
Standard Protocols
Click here to view our Standard Protocols
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