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CoraLite® Plus 488-conjugated DUSP22 Monoclonal antibody

DUSP22 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B8F1

Cat No : CL488-68032

Print datasheet

Synonyms

DUSP22, FLJ35864, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX

验证数据展示

  • IF Staining of NIH/3T3 using CL488-68032

    IF Staining of NIH/3T3 using CL488-68032

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 DUSP22 antibody (CL488-68032, Clone: 3B8F1 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 68032-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68032 targets DUSP22 in IF applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Applications IF/ICC
Tested Reactivity Human, Mouse, Rat, Rabbit
Immunogen DUSP22 fusion protein Ag9831 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name dual specificity phosphatase 22
Synonyms DUSP22, FLJ35864, JKAP, JNK stimulatory phosphatase 1, JSP 1, JSP1, LMW DSP2, LMWDSP2, MAP kinase phosphatase x, MKP x, MKPX, VHX
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC022847
Gene Symbol DUSP22
Gene ID (NCBI) 56940
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRW4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP22(Dual specificity protein phosphatase 22) is also named as JSP1, LMWDSP2, MKPX. It has been demonstrated to belong to a new subgroup of small DSPs anchored at the membranes by an N-terminal myristic acid moiety and DUSP22 play a regulatory role in the JNK or p38 MAPK pathways(PMID:17384676). It has 2 isoforms produced by alternative splicing with the molecular weight of 21 kDa and 23 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DUSP22 antibody CL488-68032Download protocol
Standard Protocols
Click here to view our Standard Protocols