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验证数据展示

  • IF Staining of SH-SY5Y using CL488-10166

    IF Staining of SH-SY5Y using CL488-10166

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 DOPA decarboxylase/DDC antibody (CL488-10166) at dilution of 1:200.

CoraLite® Plus 488-conjugated DOPA decarboxylase/DDC Polyclonal antibody

DOPA decarboxylase/DDC Polyclonal Antibody for IF/ICC
Cat No. CL488-10166

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy DDC Ready-To-Use IHC Kit. DDC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10166-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10166 targets DOPA decarboxylase/DDC in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0219

Product name: Recombinant human DDC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 121-389 aa of BC008366

Sequence: WLGKMLELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASALQEALERDKAAGLIPFFMVATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLLNGVEFADSFNFNPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQIPLGRRFRSLKMWFVFRMYGVKGLQAYIRKHVQLSHEFE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dopa decarboxylase (aromatic L-amino acid decarboxylase)
别名 DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28
计算分子量 54 kDa
观测分子量 48-50 kDa
GenBank蛋白编号BC008366
基因名称 DOPA decarboxylase
Gene ID (NCBI) 1644
RRIDAB_3672430
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20711
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DOPA decarboxylase/DDC antibody CL488-10166Download protocol
Standard Protocols
Click here to view our Standard Protocols
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