验证数据展示

WB analysis using 10166-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 10166-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 10166-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DDC antibody included
IHC staining of mouse kidney using 10166-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DDC antibody included
IHC staining of rat kidney using 10166-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DDC antibody included
IHC staining of rat kidney using 10166-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with DDC antibody included
IP experiment of mouse brain using 10166-1-AP
IP result of anti-DOPA decarboxylase/DDC (IP:10166-1-AP, 4ug; Detection:10166-1-AP 1:800) with mouse brain tissue lysate 4000ug.
IF Staining of SH-SY5Y using 10166-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DOPA decarboxylase antibody (10166-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DOPA decarboxylase/DDC Polyclonal antibody

DOPA decarboxylase/DDC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10166-1-AP

产品说明书

引用文献(10)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (10)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	SH-SY5Y cells, mouse kidney tissue, mouse brain tissue, rat kidney tissue, PC-12 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse kidney tissue, human liver cancer tissue, rat kidney tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Planning an IHC experiment? We recommend our IHCeasy DDC Ready-To-Use IHC Kit. DDC primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 5 publications below
IHC	See 7 publications below
IF	See 2 publications below

产品信息

10166-1-AP targets DOPA decarboxylase/DDC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag0219 Product name: Recombinant human DDC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 121-389 aa of BC008366 Sequence: WLGKMLELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASALQEALERDKAAGLIPFFMVATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLLNGVEFADSFNFNPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQIPLGRRFRSLKMWFVFRMYGVKGLQAYIRKHVQLSHEFE 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	dopa decarboxylase (aromatic L-amino acid decarboxylase)
别名	DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28
计算分子量	54 kDa
观测分子量	48-50 kDa
GenBank蛋白编号	BC008366
基因名称	DOPA decarboxylase
Gene ID (NCBI)	1644
RRID	AB_2088937
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P20711
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

DOPA decarboxylase (DDC), also known as aromatic l-amino acid decarboxylase, belongs to the pyridoxal-dependent aminotransferase superfamily (PMID: 38467937). DDC is an enzyme that converts levodopa into dopamine, the latter being severely depleted in LBD due to the loss of dopaminergic neurons in the substantia nigra (PMID: 28751068). DDC plays an important role in the dopaminergic system and participates in the uptake and decarboxylation of amine precursors in the peripheral tissues (PMID: 28100850). It is reported that DDC may be a novel and potential marker for the detection of peritoneal micrometastases of gastric cancer (PMID: 14760382).

实验方案

Product Specific Protocols
IF protocol for DOPA decarboxylase/DDC antibody 10166-1-AP	Download protocol
IHC protocol for DOPA decarboxylase/DDC antibody 10166-1-AP	Download protocol
IP protocol for DOPA decarboxylase/DDC antibody 10166-1-AP	Download protocol
WB protocol for DOPA decarboxylase/DDC antibody 10166-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Nature Reversing a model of Parkinson's disease with in situ converted nigral neurons. Authors - Hao Qian View Article
mouse,human	WB,IHC	Cell Signal Profile of 5-HT2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity Authors - Yuxin Zhang View Article
human	WB	Dis Model Mech A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease. Authors - Zhao-Feng Li View Article
mouse	IHC,IF	Toxicol Appl Pharmacol PMID: 21703292 Authors - Fox Donald A DA View Article
human	WB	J Transl Med Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease. Authors - Ming Ming View Article
mouse,human	WB,IHC	Toxicol Appl Pharmacol Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H+] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes. Authors - Yuxin Zhang View Article

验证数据展示

WB analysis using 10166-1-AP

WB analysis using 10166-1-AP

IHC staining of mouse kidney using 10166-1-AP

IHC staining of mouse kidney using 10166-1-AP

IHC staining of rat kidney using 10166-1-AP

IHC staining of rat kidney using 10166-1-AP

IP experiment of mouse brain using 10166-1-AP

IF Staining of SH-SY5Y using 10166-1-AP

DOPA decarboxylase/DDC Polyclonal antibody

DOPA decarboxylase/DDC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Profile of 5-HT2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity

A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.

PMID: 21703292

Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease.

Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H+] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes.