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验证数据展示

  • WB analysis using 34200-1-AP (same clone as 34200-1-PBS)

    WB analysis using 34200-1-AP (same clone as 34200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 34200-1-AP (DOLPP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 34200-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 34200-1-AP (same clone as 34200-1-PBS)

    IHC staining of human cervical cancer using 34200-1-AP (same clone as 34200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 34200-1-AP (DOLPP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34200-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 34200-1-AP (same clone as 34200-1-PBS)

    IHC staining of human urothelial carcinoma using 34200-1-AP (same clone as 34200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 34200-1-AP (DOLPP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34200-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 34200-1-AP (same clone as 34200-1-PBS)

    IF Staining of A431 using 34200-1-AP (same clone as 34200-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DOLPP1 antibody (34200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 34200-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 34200-1-AP (same clone as 34200-1-PBS)

    IF Staining of U2OS using 34200-1-AP (same clone as 34200-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using DOLPP1 antibody (34200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 34200-1-PBS in a different storage buffer formulation.

DOLPP1 Polyclonal antibody, PBS Only

DOLPP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 34200-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Dolichyl pyrophosphate phosphatase 1, Dolichyldiphosphatase 1, EC:3.6.1.43, LSFR2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

34200-1-PBS targets DOLPP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag40783

Product name: Recombinant human DOLPP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 183-238 aa of BC033686

Sequence: TQEVLTPLFPRIAAWPVSEFFLIRDTSLIPNVLWFEYTVTRAEARNRQRKLGTKLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dolichyl pyrophosphate phosphatase 1
别名 Dolichyl pyrophosphate phosphatase 1, Dolichyldiphosphatase 1, EC:3.6.1.43, LSFR2
计算分子量 238 aa, 27 kDa
观测分子量22 kDa
GenBank蛋白编号BC033686
基因名称 DOLPP1
Gene ID (NCBI) 57171
RRIDAB_3743171
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86YN1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DOLPP1 (Dolichyl Pyrophosphate Phosphatase 1) is a multiple-pass transmembrane protein localized to the endoplasmic reticulum membrane and serves as a key regulatory enzyme in the N-glycosylation pathway. This enzyme catalyzes the dephosphorylation of dolichyl pyrophosphate (Dol-PP) to generate dolichyl phosphate (Dol-P), thereby facilitating the recycling of glycosyl carrier lipids, which is essential for maintaining N-glycosylation efficiency and optimal levels of lipid-linked oligosaccharides (LLO). Deficiency of DOLPP1 leads to the accumulation of Dol-PP and depletion of Dol-P, resulting in protein glycosylation defects and endoplasmic reticulum stress. Studies have shown that DOLPP1 forms a functional network with glycosylation-related proteins such as MPDU1 and DPM1/2/3, participating in metabolic disorders associated with congenital disorders of glycosylation (CDG), and acts as an important regulator of the cellular secretory pathway and protein quality control.

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