验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, MCF-7 cells, MDA-MB-231 cells, RKO cells |
| Positive IHC detected in | human urothelial carcinoma tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34200-1-AP targets DOLPP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40783 Product name: Recombinant human DOLPP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 183-238 aa of BC033686 Sequence: TQEVLTPLFPRIAAWPVSEFFLIRDTSLIPNVLWFEYTVTRAEARNRQRKLGTKLQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dolichyl pyrophosphate phosphatase 1 |
| 别名 | Dolichyl pyrophosphate phosphatase 1, Dolichyldiphosphatase 1, EC:3.6.1.43, LSFR2 |
| 计算分子量 | 238 aa, 27 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC033686 |
| 基因名称 | DOLPP1 |
| Gene ID (NCBI) | 57171 |
| RRID | AB_3743171 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q86YN1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DOLPP1 (Dolichyl Pyrophosphate Phosphatase 1) is a multiple-pass transmembrane protein localized to the endoplasmic reticulum membrane and serves as a key regulatory enzyme in the N-glycosylation pathway. This enzyme catalyzes the dephosphorylation of dolichyl pyrophosphate (Dol-PP) to generate dolichyl phosphate (Dol-P), thereby facilitating the recycling of glycosyl carrier lipids, which is essential for maintaining N-glycosylation efficiency and optimal levels of lipid-linked oligosaccharides (LLO). Deficiency of DOLPP1 leads to the accumulation of Dol-PP and depletion of Dol-P, resulting in protein glycosylation defects and endoplasmic reticulum stress. Studies have shown that DOLPP1 forms a functional network with glycosylation-related proteins such as MPDU1 and DPM1/2/3, participating in metabolic disorders associated with congenital disorders of glycosylation (CDG), and acts as an important regulator of the cellular secretory pathway and protein quality control.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DOLPP1 antibody 34200-1-AP | Download protocol |
| IHC protocol for DOLPP1 antibody 34200-1-AP | Download protocol |
| WB protocol for DOLPP1 antibody 34200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
