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验证数据展示

  • FC experiment of Jurkat using CL594-66587

    FC experiment of Jurkat using CL594-66587

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DNAJB6 (CL594-66587, Clone:1A1D2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL594-66587

    IF Staining of HepG2 using CL594-66587

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 DNAJB6 antibody (CL594-66587, Clone: 1A1D2 ) at dilution of 1:200, CL488-Phalloidin (green).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated DNAJB6 Monoclonal antibody

DNAJB6 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66587

产品说明书

CloneNo. 1A1D2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

DJ4, DnaJ, Heat shock protein J2, HHDJ1, HSJ 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66587-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66587 targets DNAJB6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 DNAJB6 fusion protein Ag2258 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 6
别名 DJ4, DnaJ, Heat shock protein J2, HHDJ1, HSJ 2
计算分子量 241 aa, 27 kDa
观测分子量 27 kDa, 38 kDa
GenBank蛋白编号BC000177
基因名称 DNAJB6
Gene ID (NCBI) 10049
RRIDAB_3084716
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDO75190
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.

实验方案

Product Specific Protocols
IF protocol for CL594 DNAJB6 antibody CL594-66587Download protocol
Standard Protocols
Click here to view our Standard Protocols